PS125. Functional and morphological changes induced by ketamine in the hippocampus

Specific objective of the study: This study is aimed to investigate desipramine, venlafaxine and bupropion on depression and anxiety in mice. Methods used: We used a classic tricyclic antidepressant, desipramine; a serotonin-norepinephrine reuptake inhibitör, venlafaxine; a second-generation antidepressant agent that blocks centrally the reuptake of noradrenaline and dopamine, bupropion in forced swimming test (FST), elevated plus maze test (EPM) and open field test. Summary of results: Imipramine (30 mg/kg), desipramine (7.5 mg/kg and 15 mg/kg), venlafaxine (4 mg/kg and 8 mg/kg), bupropion (20 mg/kg and 40 mg/kg) significantly reduced immobility time dose dependently, compared to vehicle-treated group. And also bupropion (40 mg/kg) exerted significant antidepressant-like effects in the mice FST which was more effective than imipramine. Diazepam (2 mg/kg), desipramine (7.5 mg/kg and 15 mg/kg), venlafaxine (4 mg/kg and 8 mg/kg), bupropion (20 mg/kg and 40 mg/ kg) significantly increased the time spent in the open arms dose dependently, compared to the vehicle-treated group. Diazepam (2 mg/kg), desipramine (7.5 mg/kg and 15 mg/kg), venlafaxine 8 mg/kg) and bupropion (20 mg/kg and 40 mg/kg) significantly increased the number of entries into the open arms, compared to the vehicle-treated group. And also bupropion (40 mg/kg) exerted significant anxiolytic-like effects in the mice EPM test which was more effective than diazepam. None of the drugs modified the total distance moved in the open field test. Conclusions reached: desipramine, venlafaxine and bupropion (20 mg/kg) exerted significant antidepressant-like effects which was as effective as imipramine. And also bupropion (40 mg/kg) exerted significant antidepressant-like effects which was more effective than imipramine. Again, desipramine, venlafaxine and bupropion (20 mg/kg) exerted significant anxiolytic-like effects which was as effective as diazepam. And also bupropion (40 mg/ kg) exerted significant anxiolytic-like effects which was more effective than diazepam. PS125 Functional and morphological changes induced by ketamine in the hippocampus Giulia Treccani1,2, Nico Liebenberg N1, Sandra Tillmann1, Laura Musazzi2, Marco Milanese3, Fenghua Chen4, Betina Elfving1, Gregers Wegener1,5, Jens Randel Nyengaard4, Maurizio Popoli2, Heidi Kaastrup Müller1. 1 Translational Neuropsychiatry Unit, Department of Clinical Medicine, Aarhus University, Risskov, Denmark 2 Laboratory of Neuropsychopharmacology and Functional Neurogenomics, Dipartimento di Scienze Farmacologiche e Biomolecolari and Center of Excellence on Neurodegenerative Diseases (CEND), Università degli Studi di Milano, Milan, Italy 3 Department of Pharmacy, Unit of Pharmacology and Toxicology, University of Genova, Genoa, Italy. 4 Stereology and Electron Microscopy Laboratory, Centre for Stochastic Geometry and Advanced Bioimaging, Aarhus University Hospital, Aarhus, Denmark 5 Pharmaceutical Research Center of Excellence, School of Pharmacy, North-West University, Potchefstroom, South